• The enigma of interspecific plasmodesmata: insight from parasitic plants 

      Fischer, Karsten; Lachner, Lena Anna-Maria; Olsen, Stian; Mulisch, Maria; Krause, Kirsten (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-04-01)
      Parasitic plants live in intimate physical connection with other plants serving as their hosts. These host plants provide the inorganic and organic compounds that the parasites need for their propagation. The uptake of the macromolecular compounds happens through symplasmic connections in the form of plasmodesmata. In contrast to regular plasmodesmata, which connect genetically identical cells of ...
    • A highly efficient protocol for transforming Cuscuta reflexa based on artificially induced infection sites 

      Lachner, Lena Anna-Maria; Galstyan, Levon; Krause, Kirsten (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-08-10)
      The parasitic plant genus <i>Cuscuta</i> is notoriously difficult to transform and to propagate or regenerate in vitro. With it being a substantial threat to many agroecosystems, techniques allowing functional analysis of gene products involved in host interaction and infection mechanisms are, however, in high demand. We set out to explore whether <i>Agrobacterium</i>‐mediated transformation of ...
    • How to tame a parasite - Developing biotechnological pipelines for gene function studies in Cuscuta 

      Lachner, Lena Anna-Maria (Doctoral thesis; Doktorgradsavhandling, 2022-05-20)
      <p><i>Cuscuta</i> is a genus of parasitic plants that form cross-species bridges and connect to the xylem and phloem of a wide variety of host plants. Because of their unique lifestyle, research into processes like the formation of connections in between plant cells and plant immunity would benefit from <i>Cuscuta</i> as a model system. However, a method to study gene functions in a <i>Cuscuta</i> ...
    • Selective mineral transport barriers at Cuscuta-host infection sites 

      Förste, Frank; Mantouvalou, Ioanna; Kanngiesser, Birgit; Stosnach, Hagen; Lachner, Lena Anna-Maria; Fischer, Karsten; Krause, Kirsten (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-11)
      The uptake of inorganic nutrients by rootless parasitic plants, which depend on host connections for all nutrient supplies, is largely uncharted. Using X‐ray fluorescence spectroscopy (XRF), we analyzed the element composition of macro‐ and micronutrients at infection sites of the parasitic angiosperm <i>Cuscuta reflexa</i> growing on hosts of the genus <i>Pelargonium</i>. Imaging methods combining ...